Journal: Aging (Albany NY)

Article Title: Long-term intake of Lactobacillus paracasei KW3110 prevents age-related chronic inflammation and retinal cell loss in physiologically aged mice

doi: 10.18632/aging.101583

Figure Lengend Snippet: Intake of Lactobacillus paracasei KW3110 suppressed the inflammatory CD4-positive T cell expansion in the lamina propia of the small intestine (SI-LP). ( A and B ) To detect inflammatory cytokine-producing cells, SI-LP cells from young mice (3-months-old) and aged mice (17-months-old) were cultured under stimulation with Leukocyte Activation Cocktail plus BD GolgiPlug, and analyzed by flow cytometry. ( A ) Representative data of CD4-positive cells from aged mice fed a diet with (KW3110) or without (CTL) L. paracasei KW3110. ( B ) The ratio of CD3ε- and CD4-positive to live cells. ( C ) Representative data of CD4- and interferon gamma (IFN-γ)-positive cells from aged mice fed a diet with or without L. paracasei KW3110. ( D ) The ratio of CD4- and IFN-γ-positive cells to CD4-positive cells. ( E ) The expressions of programmed cell death protein 1 (PD-1) in CD3ε- and CD4-positive cells were analyzed by flow cytometry. M.F.I. indicates mean fluorescence intensity.

Article Snippet: Blood samples were collected into heparin-coated tubes and centrifuged at 3,000 rpm for 5 min. Supernatants were collected and analyzed for serum cytokine concentrations using a Bio-Plex Pro mouse cytokine assay kit (Bio-Rad, Hercules, CA, USA).

Techniques: Cell Culture, Activation Assay, Flow Cytometry, Fluorescence